3D Particle Tracking in Bifocal Imaging Mode
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3-Dimensional tracking of particles poses a challenge
in terms of accurately measuring the Z-axis position.
Typically it requires the acquisition of multiple images in
different focal planes (using expensive scanning devices)
as a function of time. The Z-stacks thus obtained comprise
the 4D dataset (x,y,z,t ) that the tracking information is
usually extracted from. Acquisition of Z-stacks is time-consuming
and reduces the time-resolution of the data.
The technique using the Dual View introduced by
Paul Selvin’s group at the University of Illinois at Urbana
Champaign simplifies the measurement considerably by
employing simultaneous bifocal imaging with no moving
parts.
